[[ https://www.creative-bioarray.com/immortalized-human-astrocytes-sv40t-csc-c12025z-item-45399.htm | Immortalized Human Astrocytes-SV40T ]] have been obtained immortalizing Human Cortical Astrocytes with SV40LT expressing lentiviral particles. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 5th passage while the SV40tranduced cells go beyond 30 passages.