[[ https://www.creative-bioarray.com/Human-Pulmonary-Artery-Endothelial-Cells-CSC-C8602W-item-41051.htm | Human Pulmonary Artery Endothelial Cells ]] from Creative Bioarray are isolated from human pulmonary artery. Human Pulmonary Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application ofmagnetic beads pre-coated with secondary antibody.