The Arabidopsis CRISPR/Cas9 Genome Editing Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains a sgRNA expression cassette driven by AtU6 promoter, and a codon optimized Cas9 expression cassette driven by CAMV 35S promoter. The Arabidopsis CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of dicots, especially in Arabidopsis.