Creative Biogene has created the QVirus™ Platform to rapidly generate lentiviral Cas9 constructs for multiplex gene manipulation. This platform readily enabled multiplex gene editing and transient or sustained transcriptional activation in human cells. The lentiviral CRISPR/Cas9 system is useful for both basic science and therapeutic applications by enabling highly efficient and tunable genomic manipulations in cell types that are difficult to transfect, such as primary cells and progenitor cells. Additionally, the vector will be useful for using multiplex gene editing for modeling complex diseases caused by combinations of mutations.